Herbert Irving Comprehensive Cancer Center
Flash Section
HICCC

Confocal & Specialized Microscopy

Liza Pon, Ph.D., Director

Theresa Swayne, Ph.D., Manager

Cedric Espenel, Ph.D., Manager

 

The Imaging Facility

The Confocal and Specialized Microscopy Shared Resource (CSMSR) provides advanced microscope systems for multidimensional optical imaging of living and fixed cells and tissues. The CSMSR offers a wide range of state-of-the-art microscopy technologies, from super-resolution microscopy to laser capture and microirradiation.

Our full-time managers provide assistance using the microscopes, training for independent use, and technical support. Trained in cell biology, neurobiology, and biophysics with in-depth expertise in light microscopy, we can help with experimental design, image acquisition, and data interpretation.

Technologies offered (details)

  • Confocal microscopy (scanning and spinning-disk)
  • Two-photon microscopy
  • Wide-field fluorescence microscopy
  • Digital deconvolution
  • Transmitted-light imaging (phase, DIC, histology)
  • Super-resolution imaging (STORM and SIM)
  • Cell surface imaging with <100 nM z-resolution (TIRF)
  • Laser-capture microdissection
  • Microfluidic cell culture while imaging
  • Software for image analysis, processing, and presentation

Application examples

  • Live-cell imaging
  • Fluorescence Recovery after Photobleaching (FRAP)
  • Förster Resonance Energy Transfer (FRET)
  • Colocalization analysis
  • Cell and particle tracking studies

Contact us to inquire about your experiments!

Don’t forget to acknowledge the CSMSR on your publications.

Sample text:
Images were collected and/or image processing and analysis for this work was performed in the Confocal and Specialized Microscopy Shared Resource of the Herbert Irving Comprehensive Cancer Center at Columbia University, supported by NIH grant #P30 CA013696 (National Cancer Institute).

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