Herbert Irving Comprehensive Cancer Center
Flash Section
HICCC

Flow Cytometry

Donna Farber, PhD, Director

Siu-Hong Ho, PhD, Manager

Flow cytometry has become the primary tool for the identification of cell populations according to specific parameters, and is therefore employed by an ever-growing number of biomedical scientists. The ability to design, perform and analyze data from multi-parametric flow cytometric experiments requires technical expertise but also immunological expertise to appropriately design the experiment.

The Flow Cytometry Shared Resource, operated jointly by the Herbert Irving Comprehensive Cancer Center and the Columbia Center for Translational Immunology (CCTI), provides training and access to state-of-the-art flow cytometry for biomedical investigators at the Columbia University Medical Center. Among the many documented applications, characterization and quantification of cells for surface protein, cytokine, intracellular transcription factor and signaling molecules are the most common experiments performed in the flow cytometry core facility. The Flow core operates at two sites in the Medical Center, on the 17th floor of the Black Building/Physicians & Surgeons Building and the third floor of the ICRC building.

Please contact the facility manager, Siu-Hong Ho, sh3056@cumc.columbia.edu, for any issues regarding training, assistance and access with flow including protocol design, instrument operation, data analysis, and troubleshooting. Additional information may be found at the CCTI Flow Cytometry web site.

To schedule equipment and make service requests, go to the iLab web site at https://cumc.corefacilities.org and log in with your UNI and password. Users should read the user policies before reserving equipment.

 

Analyzers

Instrument Features Location
BD Fortessa 405, 488, and 633nm lasers, 14 colors
Configuration Chart
BB1710
BD Fortessa 405, 488, 561, and 633nm, 13 colors
Configuration Chart
ICRC 333
BD LSRII UV, 405, 488, 532, 594, and 633nm lasers, 18 colors
Configuration Chart
Non-default dye filter change suggestion
BB1710
BD LSRII UV, 405, 488, and 633nm, 12 colors
Configuration Chart
ICRC 333
BD FACSCanto II 405, 488, and 633nm lasers, 8 colors
Configuration Chart
P&S17-503
BD FACS Calibur 488 and 633nm, 4 colors
Configuration Chart
ICRC 333

 

 

Cell Sorters

Instrument Features Location
BD Influx 405, 488, 561, and 633 lasers, 15 colors
Configuration Chart
Self Operation Policy
BB1710
BD Influx 405, 488, 561, and 633 lasers, 15 colors
Configuration Chart
Self Operation Policy
P&S 17-503
Aria II 405, 488, and 633nm, 9 colors
Configuration Chart
ICRC 312

 

Sample Preparation Guidelines for analyzers and sorters.

Leica DMI 6000BB: Configuration chart

Analysis Workstations
Workstations (PS17-503 and ICRC 333) are equipped with Flowjo and Microsoft Office

Acknowledgements

As the recipient of four NIH awards, we are required to acknowledge the NIH funding source in all publications (manuscripts, abstracts, presentations, etc.). Please use the below wording in every publication which results from the use of the flow core instruments.

If you only use the CCTI LSRII: Research reported in this publication was performed in the CCTI Flow Cytometry Core, supported in part by the Office of the Director, National Institutes of Health under awards S10RR027050. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

If you only use our Influx: Research reported in this publication was performed in the CCTI Flow Cytometry Core, supported in part by the Office of the Director, National Institutes of Health under awards S10OD020056. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

If you are a Cancer Center member or use equipment at the ICRC site: These studies used the resources of the Herbert Irving Comprehensive Cancer Center Flow Cytometry Shared Resources funded in part through Center Grant P30CA013696.

If you are a DERC (Diabetes and Endocrinology Research Center) member: These studies used the resources of the Diabetes and Endocrinology Research Center Flow Core Facility funded in part through Center Grant 5P30DK063608.

Investigators who do not comply with this NIH requirement may lose access to the core services. Please email us the Pubmed ID for the publication with the above acknowledgement.

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