Users who have paid the annual fee per calendar year may use the imaging equipment in the instrument room at ICRC 401A. The equipment is available on a first come, first served basis and filling the signing up sheets is mandatory. The available equipment includes a densitometer, a GE Healthcare Storm 8200 instrument and a GE Healthcare Typhoon Trio.
Storm 820 is intended for the scanning/quantitation of radioactive images of gels, Western, Southern, and Northern blots.
Typhoon applications include quantitation of ethidium bromide stained gels, high sensitivity nucleic acid and protein detection in gels and blots by chemoluminescence or fluorescence and high throughput nucleic acid quantification in 96 well plates using PicoGreen stain For more information please consult the Typhoon Trio user manual or click here for an abbreviated brochure on the instrument capabilities.
The facility has PCR machines available for quantitative analysis of nucleic acids.
Applied Biosystems 7500 Real-Time PCR System, 96-well plate five-color platform calibrated for FAMTM/SYBR® Green I, VIC®/JOE TM , NED TM/ TAMRA TM / Cy3TM, ROXTM/Texas Red®, and Cy5TM dyes.
Applied Biosystems 7500 Fast Real-Time PCR System is similar to the 7500 Real-Time PCR system plus is optimized for fast cycling, delivering results in as little as 30 minutes. Requires Fast optical plates and Fast Universal PCR mastermix.
PCR machines require advance scheduling and an account number at the time of the reservation. Click here for scheduling information.
The LightScanner system is based on Hi-Res Melting, a post PCR approach for a fast and cost effective solution for re-sequencing and mutation discovery projects. Check http://www.idahotech.com/LightScanner/ for more information and detailed protocols.
Lightscanner requires advance scheduling and an account number at the time of the reservation. Click here for scheduling information
Investigators are advised to contact the facility for initial discussions regarding the requirements for cell and tissue specimen preparations to best facilitate the required testing. Technical assistance is also provided for custom design and preparation of probes for FISH.
|Non-CC Member ($)|
|Culturing of tumor cell line and preparation of slides||75||150|
|Establishment of cell culture from rodent tumors||85||170|
|Culture, chromosome preparation, and G-banded
karyotype of human/rodent tumor cell line
|Culture, chromosome preparations, G-banded karyotype of rodent tumors||250||500|
|DNA probe labeling and testing||75||150|
|Culture, chromosome preparation and single color FISH analysis||150||300|
|Culture, chromosome preparation, and multi-color FISH analysis||200||400|
|Single Color Paraffin section FISH||125||250|
|Multi-color Paraffin Section FISH||175||350|
|Single color FISH on Tissue array||350||700|
|Multi-color FISH on Tissue array||400||800|
|Culture, chromosome preparation, and SKY on human metaphase cells||500||1000|
|Culture, chromosome preparations, and SKY on mouse metaphase cells||550||1100|
|Preparation of PAC/BAC DNA for FISH testing||125||250|