Herbert Irving Comprehensive Cancer Center
Flash Section
HICCC

Proteomics

User Fees & Policies

Modular Services
Service CC Member Non CC Member Off-campus rate Unit
Protein extraction (total lysate)  $64  $71  $89 per sample
Protein extraction (subcellular fractionation)    $131  $145  $181 per sample
Protein quantification  $39  $43  $54  
Affinity enrichment of phosphopeptides (large-scale, TiO2 or iMAC)    $214  $238  $298 per sample
In-gel digestion    $48  $53  $66 per gel band
In solution proteolysis (i.e. Trypsin)    $32  $36  $45 per sample
Sample clean up (C18 ziptip)    $80  $89  $111 per sample
TMT/iTRAQ labeling (up to 4plex)    $163  $181  $226 per sample
TMT/iTRAQ labeling (> 4plex)  $250  $278  $348 per hour
MALDI (per hour)  $181  $201  $251 per hour
Gel-based Protein Analysis
Service CC Member Non CC Member Off-campus rate Unit
ID gel band (low complexity, LC-MS/MS)  $158  $175  $219 1 digest + 2h LC-MS/MS
MRM analysis (PTM characterization, site targeted)  $347  $385  $481  
Protein profiling services
Service CC Member Non CC Member Off-campus rate Unit
Low complexity in solution digestion (label-free, e.g. IP)    $241  $268  $335 1 digest + 2h LC-MS/MS
Medium complexity sample (2 samples, label-free, e.g. fractionated sample)    $356  $396  $495 2x prepare lysate + 2 digest + 2x 4h LC-MS/MS
Medium complexity sample comparison (SILAC or SILAM)    $389  $432  $540 3x prepare lysate + 2 digest + 2x 4h LC-MS/MS
Medium complexity sample comparison (iTRAQ or TMT)    $492  $547  $684 2x prepare lysate + 2 digest + 2 plex label + 4h LC-MS/MS
High complexity sample (label-free, e.g. total lysate)    $516  $573  $716 2x prepare lysate + 2 digest + 2x 8h LC-MS/MS 
High complexity sample comparison (SILAC or SILAM)  $548  $609  $716 3x prepare lysate + 2 digest + 2x 8h LC-MS/MS
High complexity sample comparison (iTRAQ or TMT)    $585  $650  $813 2x prepare lysate + 2 digest + 2 plex label + 8h LC-MS/MS
Phosphoproteome profiling (SILA, iTRAQ or TMT)    $645  $717  $896 4x prepare lysate + 4 digest + 4 plex label + 8h LC-MS/MS
Additional Data Analysis (Statistical modeling, Protein Network analysis, etc.)
Additional data analysis $250 $278 $348 per project

 

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