Herbert Irving Comprehensive Cancer Center
Flash Section
HICCC

Proteomics

User Fees & Policies

Modular Services
Service CC Member Non CC Member Off-campus rate Unit
Protein extraction (total lysate) $45 $50 $63 per sample
Protein extraction (subcellular fractionation)    $102  $113  $141 per sample
Affinity enrichment of phosphopeptides (small-scale, TiO2 or iMAC)    $79  $88  $110 per sample
Affinity enrichment of phosphopeptides (large-scale, TiO2 or iMAC)    $158  $176  $220 per sample
In-gel digestion    $32  $35  $44 per gel band
In solution proteolysis (i.e. Trypsin)    $24  $27  $34 per sample
Sample clean up (C18 ziptip)    $51  $57  $71 per sample
TMT/iTRAQ labeling (up to 4plex)    $120  $133  $166 per sample
LC-MS/MS (increments of 1hr) high resolution    $213  $237  $296 per hour
LC-MS/MS (increments of 1hr) low resolution    $145  $161  $201 per hour
Single Protein PTM analysis (MRM)
Service CC Member Non CC Member Off-campus rate Unit
MRM analysis (PTM charactereization, site targeted) $273 $303 $379 per run (gel band, up to 2 sites)
Protein profiling services
Service CC Member Non CC Member Off-campus rate Unit
1D gel band (low complexity, LC-MS/MS)  $158  $176  $220 1 digest + 2h LC-MS/MS
Low complexity in solution digestion (label-free)    $213  $237  $296 1 digest + 2h LC-MS/MS
Medium complexity sample (2 samples, label-free)    $587 ($294*)  $653  $816 2x prepare lysate + 2 digest + 2x 4h LC-MS/MS
Medium complexity sample comparison (2 samples+SILAC standard)    $633 ($316*)  $703  $879 3x prepare lysate + 2 digest + 2x 4h LC-MS/MS
Medium complexity sample comparison (2 samples, iTRAQ or TMT)    $790 ($395*)  $877  $1096 2x prepare lysate + 2 digest + 2 plex label + 4h LC-MS/MS
High complexity sample (2 samples, label-free)    $915 ($457*)  $1016  $1270 2x prepare lysate + 2 digest + 2x 8h LC-MS/MS 
High complexity sample comparison (2 samples+SILAC standard)  $960 ($480*)  $1067  $1334 3x prepare lysate + 2 digest + 2x 8h LC-MS/MS
High complexity sample comparison (2 samples, iTRAQ or TMT)    $1047 ($523*)  $1163  $1454 2x prepare lysate + 2 digest + 2 plex label + 8h LC-MS/MS
Phosphoproteome profiling (4 samples, iTRAQ or TMT)    $2101 ($525*)  $2335  $2919 4x prepare lysate + 4 digest + 4 plex label + 8h LC-MS/MS
* cost per sample
Additional Data Analysis (Statistical modeling, Protein Network analysis, etc.)
Additional data analysis $199 $221 $276 per project

 

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